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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKFB4 Recombinant antibody (85444-3-RR)

    WB analysis using 85444-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85444-3-RR (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85444-3-RR

    Affinity and Kinetic Characterization of 85444-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85444-3-RR against Human PFKFB4 were performed. The affinity constant is 0.705 nM.

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PFKFB4 Recombinant antibody

PFKFB4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85444-3-RR
Clone No.242962H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

242962H8, 6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MOLT-4 cells, PC-3 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85444-3-RR targets PFKFB4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PFKFB4 fusion protein Ag30571 相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_001317134
Gene Symbol PFKFB4
Gene ID (NCBI) 5210
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16877
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFKFB4 antibody 85444-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols