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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKFB4 Polyclonal antibody (29902-1-AP)

    WB analysis using 29902-1-AP (same clone as 29902-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29902-1-AP (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PFKFB4 Polyclonal antibody (29902-1-AP)

    WB analysis using 29902-1-AP (same clone as 29902-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29902-1-AP (PFKFB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PFKFB4 Polyclonal antibody (29902-1-AP)

    IHC staining of mouse testis using 29902-1-AP (same clone as 29902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PFKFB4 Polyclonal antibody (29902-1-AP)

    IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PFKFB4 Polyclonal antibody (29902-1-AP)

    IHC staining of human kidney using 29902-1-AP (same clone as 29902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PFKFB4 Polyclonal antibody (29902-1-AP)

    IHC staining of mouse testis using 29902-1-AP (same clone as 29902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29902-1-AP (PFKFB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using PFKFB4 Polyclonal antibody (29902-1-AP)

    IF Staining of HUVEC using 29902-1-AP (same clone as 29902-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using PFKFB4 antibody (29902-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29902-1-PBS in a different storage buffer formulation.

PFKFB4 Polyclonal antibody, PBS Only

PFKFB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29902-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

6PF 2 K/Fru 2,6 P2ase 4, PFK/FBPase 4

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29902-1-PBS targets PFKFB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30571

Product name: Recombinant human PFKFB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 139-232 aa of NM_001317134

Sequence: ATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYVNRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVAD

相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
Calculated molecular weight 54 kDa
Observed molecular weight54 kDa
GenBank accession numberNM_001317134
Gene Symbol PFKFB4
Gene ID (NCBI) 5210
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16877
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4), is a bi-function enzyme. On one hand, PFKFB4 synthesizes fructose 2,6-bisphosphate (F2,6-BP) to stimulate glycolysis, playing important roles in metabolic processes. On the other hand, PFKFB4 acts as a phosphatase to hydrolyze F2,6-BP into fructose-6-phosphate (F6P) and inorganic phosphate (Pi), functioning as a protein kinase to phosphorylate protein substrates. The dual function of PFKFB4 makes it involved in multiple biological processes such as cell cycle regulation, cell proliferation and transcriptional regulation(PMID: 33593309). PFKFB4 has been reported to be involved in a variety of cancers including human bladder cancer, gastric cancer, colon cancer, breast cancer and other malignant tumors(PMID: 21396842, PMID: 33732372, PMID: 31244553).

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