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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKFB3 Recombinant monoclonal antibody (87233-1-RR)

    WB analysis using 87233-1-RR (same clone as 87233-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87233-1-RR (PFKFB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87233-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat thymus tissue using PFKFB3 Recombinant monoclonal antibody (87233-1-RR)

    WB analysis of rat thymus using 87233-1-RR (same clone as 87233-1-PBS)

    Rat thymus tissue were subjected to SDS PAGE followed by western blot with 87233-1-RR (PFKFB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87233-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PFKFB3 Recombinant monoclonal antibody (87233-1-RR)

    IF Staining of A549 using 87233-1-RR (same clone as 87233-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PFKFB3 antibody (87233-1-RR, Clone: 252490B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87233-1-PBS in a different storage buffer formulation.

  • Affinity of 87233-1-RR

    Affinity of 87233-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87233-1-RR against Human PFKFB3 were performed. The affinity constant is 0.227 nM.

PFKFB3 Recombinant monoclonal antibody, PBS Only

PFKFB3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87233-1-PBS
Clone No.252490B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

6PF-2-K/Fru-2,6-P2ase 3, 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme, 6-phosphofructo-2-kinase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, Fructose-2,6-bisphosphatase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87233-1-PBS targets PFKFB3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4744

Product name: Recombinant human PFKFB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 243-520 aa of BC040482

Sequence: HVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Calculated molecular weight 520 aa, 60 kDa
Observed molecular weight58 kDa
GenBank accession numberBC040482
Gene Symbol PFKFB3
Gene ID (NCBI) 5209
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16875
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PFKFB3, also named as NY-REN-56 and iPFK-2, plays a role in glucose metabolism. Its synthesis and degradation of fructose 2,6-bisphosphate. Endogenously generated adenosine cooperates with bacterial components to increase PFKFB3 isozyme activity, resulting in greater fructose 2,6-bisphosphate accumulation. PFKFB3 is required for increased growth, metabolic activity and is regulated through active JAK2 and STAT5.

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