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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PFKFB1 Polyclonal antibody (21718-1-AP)

    WB analysis using 21718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21718-1-AP (PFKFB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PFKFB1 Polyclonal antibody (21718-1-AP)

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PFKFB1 Polyclonal antibody (21718-1-AP)

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PFKFB1 Polyclonal antibody

PFKFB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21718-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, SMM-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21718-1-AP targets PFKFB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16362

Product name: Recombinant human PFKFB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 138-241 aa of BC096079

Sequence: ERRSLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHIQSRTV

相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Calculated molecular weight 471 aa, 55 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC096079
Gene Symbol PFKFB1
Gene ID (NCBI) 5207
RRIDAB_2878911
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP16118
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFKFB1 antibody 21718-1-APDownload protocol
IHC protocol for PFKFB1 antibody 21718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols