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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PFKFB1 Polyclonal antibody

PFKFB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21718-1-AP

Synonyms

6PF 2 K/Fru 2,6 P2ase 1, F6PK, HL2K, PFK/FBPase 1, PFKFB1, PFRX

Validation Data Gallery

Applications:
  • WB analysis using 21718-1-AP

    WB analysis using 21718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21718-1-AP (PFKFB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21718-1-AP

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 21718-1-AP

    IHC staining of human liver cancer using 21718-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21718-1-AP (PFKFB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, SMM-7721 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21718-1-AP is PFKFB1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PFKFB1 fusion protein Ag16362 相同性解析による交差性が予測される生物種
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Calculated molecular weight 471 aa, 55 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC096079
Gene symbol PFKFB1
Gene ID (NCBI) 5207
RRIDAB_2878911
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PFKFB1 antibody 21718-1-APDownload protocol
IHC protocol for PFKFB1 antibody 21718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols