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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CXCL4/PF4 Polyclonal antibody (21157-1-AP)

    WB analysis of human peripheral blood platelets using 21157-1-AP (same clone as 21157-1-PBS)

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using CXCL4/PF4 Polyclonal antibody (21157-1-AP)

    WB analysis of mouse spleen using 21157-1-AP (same clone as 21157-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse serum using CXCL4/PF4 Polyclonal antibody (21157-1-AP)

    IP experiment of mouse serum using 21157-1-AP (same clone as 21157-1-PBS)

    IP result of anti-CXCL4/PF4 (IP:21157-1-AP, 4ug; Detection:21157-1-AP 1:1000) with mouse serum lysate 2200 ug. This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CXCL4/PF4 Polyclonal antibody (21157-1-AP)

    IHC staining of mouse spleen using 21157-1-AP (same clone as 21157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21157-1-AP (CXCL4/PF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CXCL4/PF4 Polyclonal antibody (21157-1-AP)

    IF Staining of HeLa using 21157-1-AP (same clone as 21157-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PF4 antibody (21157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21157-1-PBS in a different storage buffer formulation.

CXCL4/PF4 Polyclonal antibody, PBS Only

CXCL4/PF4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21157-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21157-1-PBS targets CXCL4/PF4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12345

Product name: Recombinant human PF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-101 aa of BC093965

Sequence: MSSAAGFCASRPGLLFLGLLLLPLVVAFASAEAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

相同性解析による交差性が予測される生物種
Full Name platelet factor 4
Calculated molecular weight 101 aa, 11 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC093965
Gene Symbol PF4
Gene ID (NCBI) 5196
RRIDAB_2878819
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP02776
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The chemokine (C-X-C motif) ligand 4 (CXCL4) is also named as PF4 (Platelet factor-4) and SCYB4. The platelets, the most abundant source of CXCL4 in vivo, control profibrotic macrophage activation via CXCL4. (PMID: 36807143). Exogenous CXCL4 drove profibrotic activation of monocyte-derived dendritic cells in vitro (PMID: 33042127). Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.

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