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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PET117 Polyclonal antibody (31066-1-AP)

    WB analysis using 31066-1-AP (same clone as 31066-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 31066-1-AP (PET117 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PET117 Polyclonal antibody (31066-1-AP)

    IHC staining of human kidney using 31066-1-AP (same clone as 31066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 31066-1-AP (PET117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31066-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PET117 Polyclonal antibody (31066-1-AP)

    IHC staining of human liver using 31066-1-AP (same clone as 31066-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31066-1-AP (PET117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31066-1-PBS in a different storage buffer formulation.

PET117 Polyclonal antibody, PBS Only

PET117 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31066-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CSRP2BP, MC4DN1, PET117 Homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31066-1-PBS targets PET117 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34525

Product name: Recombinant human PET117 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-81 aa of NX_Q6UWS5

Sequence: VHVKQQWDQQRLRDGVIRDIERQIRKKENIRLLGEQIILTEQLEAEREKMLLAKGSQKS

相同性解析による交差性が予測される生物種
Full Name Protein PET117 homolog, mitochondrial
Calculated molecular weight81aa, 9 kDa
Observed molecular weight10kDa
GenBank accession numberNX_Q6UWS5
Gene Symbol PET117
Gene ID (NCBI) 100303755
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6UWS5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PET117 is a chaperone protein involved in complex IV assembly that plays a role in the regulation of mitochondria-encoded cytochrome c oxidase 1 (COX1) protein synthesis in human cells (PMID: 37247699).

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