Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, L02 cells, Y79 cells, BxPC-3 cells, K-562 cells, HL-60 cells, THP-1 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 18 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
67513-1-Ig targets PER2 (isoform 1) in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29489 Product name: Recombinant human PER2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 740-839 aa of NM_022817 Sequence: SFLQKFKEIRKLSIFQSHCHYYLQERSKGQPSERTAPGLRNTSGIDSPWKKTGKNRKLKSKRVKPRDSSESTGSGGPVSARPPLVGLNATAWSPSDTSQS 相同性解析による交差性が予測される生物種 |
| Full Name | period homolog 2 (Drosophila) |
| Calculated molecular weight | 137 kDa |
| Observed molecular weight | 150~160 kDa |
| GenBank accession number | NM_022817 |
| Gene Symbol | PER2 |
| Gene ID (NCBI) | 8864 |
| RRID | AB_2882734 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15055 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, and 67513-1-Ig detected molecular weight of 150-160 kDa (PMID:16675517), while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PER2 (isoform 1) antibody 67513-1-Ig | Download protocol |
| WB protocol for PER2 (isoform 1) antibody 67513-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Phytomedicine Ji-Ming-San enhances intestinal circadian rhythms and mitigates colitis in mice: The role of epithelial RORα | ||
Sci Total Environ Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario | ||
Cell Chem Biol Lactylation of mTOR enhances autophagy in skeletal muscle during exercise. | ||
Free Radic Biol Med BMAL1-mediated circadian-ferroptosis crosstalk drives neuronal vulnerability after TBI. | ||
Neuropsychopharmacology The crosstalk between CREB and PER2 mediates the transition between mania- and depression-like behavior | ||
CNS Neurosci Ther Cellular iron depletion enhances behavioral rhythm by limiting brain Per1 expression in mice |