Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells |
| Positive IF/ICC detected in | MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29954-1-AP targets PER2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31471 Product name: Recombinant human PER2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of NM_022817 Sequence: MNGYAEFPPSPSNPTKEPVEPQPSQVPLQEDVDMSSGSSGHETNENCSTGRDSQGSDCDDSGKELGMLVEPPDARQSPDTFSLMMAKSEHNPSTSGCSSDQSSKVDTHKELIKTLKELKVHLPADKKAKGKASTLATLKYALRSVKQVKA 相同性解析による交差性が予測される生物種 |
| Full Name | period homolog 2 (Drosophila) |
| Calculated molecular weight | 137KD |
| Observed molecular weight | 45 kDa |
| GenBank accession number | NM_022817 |
| Gene Symbol | PER2 |
| Gene ID (NCBI) | 8864 |
| RRID | AB_3086198 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15055 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PER2, also named as KIAA0347, is a component of the circadian clock mechanism which is essential for generating circadian rhythms. Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) which is characterized by very early sleep onset and offset. There are two isoforms of PER2: isoform 1 has an expected molecular size of about 140 kDa, while isoform 2, known as PER2S (a second splicing variant of the human PER2 gene), has a molecular weight of about 45 kDa (PMID:26347774). 29954-1-AP detected molecular weight of 45 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PER2 antibody 29954-1-AP | Download protocol |
| WB protocol for PER2 antibody 29954-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
