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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using PEMT Recombinant antibody (85099-1-RR)

    WB analysis of SH-SY5Y using 85099-1-RR (same clone as 85099-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    WB analysis of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 85099-1-RR (PEMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PEMT Recombinant antibody (85099-1-RR)

    IHC staining of mouse liver using 85099-1-RR (same clone as 85099-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85099-1-RR (PEMT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85099-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85099-1-RR

    Affinity and Kinetic Characterization of 85099-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85099-1-RR against Human PEMT were performed. The affinity constant is 0.379 nM.

PEMT Recombinant monoclonal antibody, PBS Only

PEMT Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85099-1-PBS
Clone No.242150D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Phosphatidylethanolamine N-methyltransferase, PEMT2, PEMPT, PEAMT, EC:2.1.1.17

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85099-1-PBS targets PEMT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8034

Product name: Recombinant human PEMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-236 aa of BC050593

Sequence: MKRSGNPGAEVTNSSVAGPDCCGGLGNIDFRQADFCVMTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTILLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGDYFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYEEPFTAEIYRQKASGSHKRS

相同性解析による交差性が予測される生物種
Full Name phosphatidylethanolamine N-methyltransferase
Calculated molecular weight 26 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC050593
Gene Symbol PEMT
Gene ID (NCBI) 10400
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9UBM1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PEMT Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PMID:12431977, 15927961).

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