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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PELO Recombinant antibody (85743-1-RR)

    WB analysis using 85743-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85743-1-RR (PELO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PELO Recombinant antibody (85743-1-RR)

    IF Staining of HeLa using 85743-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PELO antibody (85743-1-RR, Clone: 243137F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85743-1-RR

    Affinity and Kinetic Characterization of 85743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85743-1-RR against Human PELO were performed. The affinity constant is below 1 pM.

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PELO Recombinant antibody

PELO Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85743-1-RR
Clone No.243137F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, MDA-MB-231 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85743-1-RR targets PELO in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PELO fusion protein Ag0873 相同性解析による交差性が予測される生物種
Full Name pelota homolog (Drosophila)
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC005889
Gene Symbol PELO
Gene ID (NCBI) 53918
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BRX2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

Protocols

Product Specific Protocols
WB protocol for PELO antibody 85743-1-RRDownload protocol
IF protocol for PELO antibody 85743-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols