Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10582-1-AP (same clone as 10582-1-PBS)
Various cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
WB analysis using 10582-1-AP (same clone as 10582-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 10582-1-AP (same clone as 10582-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)
IP result of anti-PELO (IP:10582-1-AP, 4ug; Detection:10582-1-AP 1:500) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of mouse pancreas using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of rat ovary using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of mouse stomach using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IHC staining of mouse ovary using 10582-1-AP (same clone as 10582-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 10582-1-AP (same clone as 10582-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PELO antibody (10582-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10582-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PELO Polyclonal antibody, PBS Only

PELO Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10582-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10582-1-PBS targets PELO in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0873 Product name: Recombinant human PELO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 42-384 aa of BC005889 Sequence: STIRKVQTESSTGSVGSNRVRTTLTLCVEAIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAVVMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASPGFVREQFCDYMFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDDFYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLHVSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEE 相同性解析による交差性が予測される生物種
Full Name	pelota homolog (Drosophila)
Calculated molecular weight	43 kDa
Observed molecular weight	43-45 kDa
GenBank accession number	BC005889
Gene Symbol	PELO
Gene ID (NCBI)	53918
RRID	AB_2236833
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BRX2
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

WB analysis using 10582-1-AP (same clone as 10582-1-PBS)

WB analysis of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

WB analysis of HeLa using 10582-1-AP (same clone as 10582-1-PBS)

WB analysis of mouse testis using 10582-1-AP (same clone as 10582-1-PBS)

IP experiment of HEK-293 using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of human liver using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of human ovary cancer using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of mouse pancreas using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of rat ovary using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of mouse stomach using 10582-1-AP (same clone as 10582-1-PBS)

IHC staining of mouse ovary using 10582-1-AP (same clone as 10582-1-PBS)

IF Staining of HeLa using 10582-1-AP (same clone as 10582-1-PBS)