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Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using PELI1 Polyclonal antibody (12053-1-AP)

    WB analysis of THP-1 using 12053-1-AP (same clone as 12053-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 12053-1-AP (PELI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using PELI1 Polyclonal antibody (12053-1-AP)

    IP experiment of SH-SY5Y using 12053-1-AP (same clone as 12053-1-PBS)

    IP result of anti-PELI1 (IP:12053-1-AP, 4ug; Detection:12053-1-AP 1:300) with SH-SY5Y cells lysate 3000ug. This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PELI1 Polyclonal antibody (12053-1-AP)

    IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PELI1 Polyclonal antibody (12053-1-AP)

    IHC staining of mouse brain using 12053-1-AP (same clone as 12053-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12053-1-AP (PELI1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12053-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12053-1-PBS targets PELI1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PELI1 fusion protein Ag2683 相同性解析による交差性が予測される生物種
Full Name pellino homolog 1 (Drosophila)
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight 46-53 kDa
GenBank accession numberBC011419
Gene Symbol PELI1
Gene ID (NCBI) 57162
RRIDAB_2161180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FA3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.