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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using MAP17 / PDZK1IP1 Polyclonal antibody (12518-1-AP)

    IHC staining of human kidney using 12518-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12518-1-AP (MAP17 / PDZK1IP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAP17 / PDZK1IP1 Polyclonal antibody (12518-1-AP)

    IHC staining of human kidney using 12518-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12518-1-AP (MAP17 / PDZK1IP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using MAP17 / PDZK1IP1 Polyclonal antibody (12518-1-AP)

    IF Staining of PC-3 using 12518-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using MAP17 / PDZK1IP1 antibody (12518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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MAP17 / PDZK1IP1 Polyclonal antibody

MAP17 / PDZK1IP1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12518-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

Protein DD96, PDZK1IP1, PDZK1-interacting protein 1, MAP17, 17 kDa membrane-associated protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12518-1-AP targets MAP17 / PDZK1IP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP17 / PDZK1IP1 fusion protein Ag3206 相同性解析による交差性が予測される生物種
Full Name PDZK1 interacting protein 1
Calculated molecular weight 114 aa, 12 kDa
GenBank accession numberBC012303
Gene Symbol MAP17
Gene ID (NCBI) 10158
RRIDAB_3085395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13113
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDZK1-interacting protein 1(PDZK1IP1), also known as MAP17, is a membrane-associated protein. MAP17 / PDZK1IP1 is overexpressed in a variety of human carcinomas, and overexpression of MAP17 / PDZK1IP1 protein is strongly correlated with the progression of prostate and ovarian carcinomas (PMID: 17426052). MAP17 / PDZK1IP1 has been proven to be an oncogene for tumorigenesis and development of papillary thyroid cancer (PTC) (PMID: 36057192; PMID: 35727731).

Protocols

Product Specific Protocols
IHC protocol for MAP17 / PDZK1IP1 antibody 12518-1-APDownload protocol
IF protocol for MAP17 / PDZK1IP1 antibody 12518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols