Validation Data Gallery
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Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
25512-1-AP targets PDZD8 in WB, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PDZD8 fusion protein Ag21914 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ domain containing 8 |
| Calculated molecular weight | 1154 aa, 129 kDa |
| Observed molecular weight | 150-160 kDa |
| GenBank accession number | BC028375 |
| Gene Symbol | PDZD8 |
| Gene ID (NCBI) | 118987 |
| RRID | AB_2880110 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDZD8 (PDZ domain containing 8) is an SMP (synaptotagmin-like mitochondrial-lipid-binding) domain-containing protein belonging to the tubular lipid-binding protein (TULIP) superfamily of lipid transfer proteins (PMID: 37961523). PDZD8 is an integral ER transmembrane protein present at ER-mitochondria contacts (PMID: 29097544). It is involved in regulating ER-mitochondria associations, mitochondrial calcium ion homeostasis, and lipid transport between the ER and late endosomes/lysosomes (PMID: 37247681; 29097544; 33912962).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDZD8 antibody 25512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Synth Biol Development of a Signal-integrating Reporter to Monitor Mitochondria-ER Contacts | ||
Nat Commun ER-mitochondria contacts mediate lipid radical transfer via RMDN3/PTPIP51 phosphorylation to reduce mitochondrial oxidative stress |