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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PDZ-GEF2 Polyclonal antibody (24583-1-AP)

    WB analysis of mouse brain using 24583-1-AP (same clone as 24583-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24583-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDZ-GEF2 Polyclonal antibody (24583-1-AP)

    WB analysis using 24583-1-AP (same clone as 24583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24583-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PDZ-GEF2 Polyclonal antibody (24583-1-AP)

    IHC staining of mouse brain using 24583-1-AP (same clone as 24583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24583-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PDZ-GEF2 Polyclonal antibody (24583-1-AP)

    IHC staining of mouse brain using 24583-1-AP (same clone as 24583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24583-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PDZ-GEF2 Polyclonal antibody (24583-1-AP)

    IHC staining of rat brain using 24583-1-AP (same clone as 24583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24583-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PDZ-GEF2 Polyclonal antibody (24583-1-AP)

    IHC staining of rat brain using 24583-1-AP (same clone as 24583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24583-1-AP (PDZ-GEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24583-1-PBS in a different storage buffer formulation.

PDZ-GEF2 Polyclonal antibody, PBS Only

PDZ-GEF2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24583-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24583-1-PBS targets PDZ-GEF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20325

Product name: Recombinant human PDZ-GEF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC142964

Sequence: MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREHQLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGKRGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEVNSYL

相同性解析による交差性が予測される生物種
Full Name Rap guanine nucleotide exchange factor (GEF) 6
Calculated molecular weight 1601 aa, 179 kDa
Observed molecular weight179 kDa
GenBank accession numberBC142964
Gene Symbol RAPGEF6
Gene ID (NCBI) 51735
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEU7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RAPGEF6, also named PDZ-GEF2 enables several functions, including GTP-dependent protein binding activity; guanyl-nucleotide exchange factor activity; and phosphatidic acid binding activity. It is also involved in microvillus assembly; positive regulation of GTPase activity; and protein localization to plasma membrane. Located in several cellular components, including apical plasma membrane; centrosome; and endocytic vesicle.

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