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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDS5A Monoclonal antibody (67520-1-Ig)

    WB analysis using 67520-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67520-1-Ig (PDS5A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PDS5A Monoclonal antibody (67520-1-Ig)

    WB analysis of HeLa using 67520-1-Ig

    WB result of PDS5A antibody (67520-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDS5A transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDS5A Monoclonal antibody (67520-1-Ig)

    IHC staining of human breast cancer using 67520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDS5A Monoclonal antibody (67520-1-Ig)

    IHC staining of human breast cancer using 67520-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67520-1-Ig (PDS5A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PDS5A Monoclonal antibody (67520-1-Ig)

    IF Staining of A431 using 67520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDS5A Monoclonal antibody (67520-1-Ig)

    IF Staining of HepG2 using 67520-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDS5A antibody (67520-1-Ig, Clone: 1C8D4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  • Featured Product
  • KD/KO Validated

PDS5A Monoclonal antibody

PDS5A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67520-1-Ig
Clone No.1C8D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SCC 112, PIG54, PDS5, KIAA0648, Cell proliferation-inducing gene 54 protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67520-1-Ig targets PDS5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PDS5A fusion protein Ag11525 相同性解析による交差性が予測される生物種
Full Name PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae)
Calculated molecular weight 1297 aa, 147 kDa
Observed molecular weight130 kDa
GenBank accession numberBC009650
Gene Symbol PDS5A
Gene ID (NCBI) 23244
RRIDAB_2882740
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ29RF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDS5A antibody 67520-1-IgDownload protocol
IHC protocol for PDS5A antibody 67520-1-IgDownload protocol
IF protocol for PDS5A antibody 67520-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols