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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PDLIM7 Polyclonal antibody (17080-1-AP)

    WB analysis of HeLa using 17080-1-AP

    WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PDLIM7 Polyclonal antibody (17080-1-AP)

    WB analysis using 17080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PDLIM7 Polyclonal antibody (17080-1-AP)

    IP experiment of HeLa using 17080-1-AP

    IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:5000) with HeLa cells lysate 1200 ug.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of mouse skeletal muscle using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human colon using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of mouse skeletal muscle using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human spleen using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human spleen using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human ovary using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human ovary using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human kidney using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human kidney using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human skin using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human skin using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PDLIM7 Polyclonal antibody (17080-1-AP)

    IHC staining of human liver using 17080-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  • Featured Product
  • KD/KO Validated

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17080-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

ENIGMA, LIM mineralization protein, LMP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human colon, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17080-1-AP targets PDLIM7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDLIM7 fusion protein Ag1334 相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 7 (enigma)
Calculated molecular weight 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC014521
Gene Symbol PDLIM7
Gene ID (NCBI) 9260
RRIDAB_2161788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDLIM7 antibody 17080-1-APDownload protocol
IHC protocol for PDLIM7 antibody 17080-1-APDownload protocol
IP protocol for PDLIM7 antibody 17080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols