Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 10221-1-AP
WB result of PDLIM7 antibody (10221-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.
WB analysis of SKOV-3 using 10221-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10221-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10221-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 10221-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 10221-1-AP
IP result of anti-PDLIM7 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:20000) with MCF-7 cells lysate 1000 ug.
IP experiment of MCF-7 using 10221-1-AP
IP result of anti-PDLIM7 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:300) with MCF-7 cells lysate 2000ug.
IHC staining of human colon using 10221-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10221-1-AP (PDLIM7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10221-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10221-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 25x lens).
IF Staining of SKOV-3 using 10221-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using PDLIM7 antibody (10221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10221-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ENIGMA, LIM mineralization protein, LMP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SKOV-3 cells, HeLa cells, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human colon tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SKOV-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 11 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10221-1-AP targets PDLIM7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0264 Product name: Recombinant human PDLIM7,LMP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 3-289 aa of BC001093 Sequence: SFKVVLEGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGSLTHIEAQNKIRACGERLSLGLSRAQPVQSKPQKASAPAADPPRYTFAPSVSLNKTARPFGAPPPADSAPQQNGQPLRPLVPDASKQRLMENTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPAPASSTPQEPWPGPTAPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQSRTSIVQAAAGGVPGGGSNNGKTPVCHQCHKVI 相同性解析による交差性が予測される生物種
Full Name	PDZ and LIM domain 7 (enigma)
Calculated molecular weight	55/60 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC001093
Gene Symbol	PDLIM7
Gene ID (NCBI)	9260
RRID	AB_2161789
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NR12
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

Protocols

Product Specific Protocols
IF protocol for PDLIM7 antibody 10221-1-AP	Download protocol
IHC protocol for PDLIM7 antibody 10221-1-AP	Download protocol
IP protocol for PDLIM7 antibody 10221-1-AP	Download protocol
WB protocol for PDLIM7 antibody 10221-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	EMBO Rep Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification. Authors - Alon Abend View Article
mouse,human	WB,IP,IHC	Cell Biosci TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1 Authors - Zonglong Wu View Article
human	IF	J Bone Miner Res LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity. Authors - Hehai Pan View Article
human	WB	Front Immunol PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB. Authors - Aya Jodo View Article
human	IHC	J Endod Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions. Authors - Wang Xiaoying X View Article
human	WB	J Orthop Res LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression. Authors - Hui Liu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 10221-1-AP

WB analysis of SKOV-3 using 10221-1-AP

WB analysis of HeLa using 10221-1-AP

WB analysis of MCF-7 using 10221-1-AP

WB analysis of SKOV-3 using 10221-1-AP

IP experiment of MCF-7 using 10221-1-AP

IP experiment of MCF-7 using 10221-1-AP

IHC staining of human colon using 10221-1-AP

IHC staining of human ovary tumor using 10221-1-AP

IF Staining of SKOV-3 using 10221-1-AP

PDLIM7 Polyclonal antibody

PDLIM7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification.

TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1

LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity.

PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.

Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.

LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.