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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDLIM4 Polyclonal antibody (18543-1-AP)

    WB analysis using 18543-1-AP (same clone as 18543-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18543-1-AP (PDLIM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using PDLIM4 Polyclonal antibody (18543-1-AP)

    WB analysis of A431 using 18543-1-AP (same clone as 18543-1-PBS)

    WB result of PDLIM4 antibody (18543-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM4 transfected A431 cells. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using PDLIM4 Polyclonal antibody (18543-1-AP)

    IHC staining of human skin cancer using 18543-1-AP (same clone as 18543-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18543-1-AP (PDLIM4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PDLIM4 Polyclonal antibody (18543-1-AP)

    IF Staining of U2OS using 18543-1-AP (same clone as 18543-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PDLIM4 antibody (18543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDLIM4 Polyclonal antibody, PBS Only

PDLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18543-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PDZ and LIM domain 4, Reversion-induced LIM protein, RIL

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18543-1-PBS targets PDLIM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13424

Product name: Recombinant human PDLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC016765

Sequence: MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRGPSGTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSRDCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERLYCESHAKARVKPPEGYDVVAVYPNAKVELV

相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 4
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC016765
Gene Symbol PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3085565
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP50479
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).

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