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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using PDLIM3 Polyclonal antibody (13199-2-AP)

    WB analysis of human heart using 13199-2-AP (same clone as 13199-2-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using PDLIM3 Polyclonal antibody (13199-2-AP)

    WB analysis of mouse spleen using 13199-2-AP (same clone as 13199-2-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13199-2-AP (PDLIM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PDLIM3 Polyclonal antibody (13199-2-AP)

    IHC staining of human stomach cancer using 13199-2-AP (same clone as 13199-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13199-2-AP (PDLIM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13199-2-PBS in a different storage buffer formulation.

PDLIM3 Polyclonal antibody, PBS Only

PDLIM3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13199-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ALP, Actinin associated LIM protein, Actinin-associated LIM protein, Alpha-actinin-2-associated LIM protein, PDZ and LIM domain 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13199-2-PBS targets PDLIM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3934

Product name: Recombinant human PDLIM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-316 aa of BC027870

Sequence: MPQTVILPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAAAHQLCLKIDRGETHLWSPQVSEDGKAHPFKINLESEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPSSPQNEPTASVPPESDVYRMLHDNRNEPTQPRQSGSFRVLQGMVDDGSDDRPAGTRSVRAPVTKVHGGSGGAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFIEGELYCETHARARTKPPEGYDTVTLYPKA

相同性解析による交差性が予測される生物種
Full Name PDZ and LIM domain 3
Calculated molecular weight 316 aa, 34 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC027870
Gene Symbol PDLIM3
Gene ID (NCBI) 27295
RRIDAB_2161653
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GG5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDLIM3, also known as ALP, contains a PDZ domain and a LIM domain. PDLIM3 binds the spectrin-like repeats of alpha-actinin-2 and colocalizes with alpha-actinin-2 at the Z lines of skeletal muscle.

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