Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, Reh cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11574-1-AP targets PDLIM2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2155 Product name: Recombinant human PDLIM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC021556 Sequence: MALTVDVAGPAPWGFRITGGRDFHTPIMVTKVAERGKAKDADLRPGDIIVAINGESAEGMLHAEAQSKIRQSPSPLRLQLDRSQATSPGQTNGDSSLEVLATRFQGSVRTYTESQSSLRSSYSSPTSLSPRAGSPFSPPPSSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQAGLGRAGDSAVLVLPPSPGPRSSRPSMDSEGGSLLLDEDSEVFKMLQENREGRAAPRQSSSFRLLQEALEAEERGGTPAFLPSSLSPQSSLPASRALATPPKLHTCEKCSTSIANQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWVGDELYCEKHARQRYSAPATLSSRA 相同性解析による交差性が予測される生物種 |
| Full Name | PDZ and LIM domain 2 (mystique) |
| Calculated molecular weight | 37.5 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC021556 |
| Gene Symbol | PDLIM2 |
| Gene ID (NCBI) | 64236 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JY6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The PDZ and LIM domain-containing protein 2(PDLIM2), also known as Mystique or SLIM, is classified as a member of the actinin-associated LIM family of proteins. PDLIM2 is an IGF-IR-regulated adapter protein located at the actin cytoskeleton and necessary for the migratory capacity of epithelial cells (PMID: 15659642, 34571051). PDLIM2 is a nuclear ubiquitin E3 ligase that negatively regulates STAT signaling(PMID: 15963787).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDLIM2 antibody 11574-1-AP | Download protocol |
| WB protocol for PDLIM2 antibody 11574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |