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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDK4 Polyclonal antibody (12949-1-AP)

    WB analysis using 12949-1-AP (same clone as 12949-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDK4 Polyclonal antibody (12949-1-AP)

    WB analysis using 12949-1-AP (same clone as 12949-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of rat heart using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PDK4 Polyclonal antibody (12949-1-AP)

    IHC staining of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDK4 Polyclonal antibody, PBS Only

PDK4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12949-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, EC:2.7.11.2, PDHK4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12949-1-PBS targets PDK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3629

Product name: Recombinant human PDK4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 61-412 aa of BC040239

Sequence: LPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTLIKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPLRIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDAIIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM

相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase kinase, isozyme 4
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC040239
Gene Symbol PDK4
Gene ID (NCBI) 5166
RRIDAB_2161499
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16654
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

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