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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDIR Polyclonal antibody (29593-1-AP)

    WB analysis using 29593-1-AP (same clone as 29593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29593-1-AP (PDIR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDIR Polyclonal antibody (29593-1-AP)

    WB analysis using 29593-1-AP (same clone as 29593-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29593-1-AP (PDIR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using PDIR Polyclonal antibody (29593-1-AP)

    IP experiment of mouse liver using 29593-1-AP (same clone as 29593-1-PBS)

    IP result of anti-PDIR (IP:29593-1-AP, 4ug; Detection:29593-1-AP 1:5000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDIR Polyclonal antibody (29593-1-AP)

    IHC staining of human placenta using 29593-1-AP (same clone as 29593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29593-1-AP (PDIR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PDIR Polyclonal antibody (29593-1-AP)

    IHC staining of human placenta using 29593-1-AP (same clone as 29593-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29593-1-AP (PDIR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29593-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDIR Polyclonal antibody, PBS Only

PDIR Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 29593-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29593-1-PBS targets PDIR in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag31479

Product name: Recombinant human PDIA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-170 aa of BC001625

Sequence: SSAKVSSLIERISDPKDLKKLLRTRNNVLVLYSKSEVAAENHLRLLSTVAQAVKGQGTICWVDCGDAESRKLCKKMKVDLSPKDKKVELFHYQDGAFHTEYNRAVTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLDSEKDFRRLLKKEE

相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 5
Calculated molecular weight 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC001625
Gene Symbol PDIR
Gene ID (NCBI) 10954
RRIDAB_2918329
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14554
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDIR(Protein disulfide isomerase-related protein), also known as PDIA5 (Protein Disulfide Isomerase Family A Member 5), is a member of the PDI gene family and also exhibits chaperone-like activity. PDIR protein is correlated with immune infiltration, which is highly expressed in glioma and participates in glioma progression(PMID: 33664747). PDIR contributes to disulfide bond rearrangement in ATF6α under stress conditions that necessary for ATF6α activation upon ER stress(PMID: 24636989).

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