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Validation Data Gallery

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  • Western Blot (WB) analysis of rat spleen tissue using PDIK1L Polyclonal antibody (12646-1-AP)

    WB analysis of rat spleen using 12646-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 12646-1-AP (PDIK1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDIK1L Polyclonal antibody (12646-1-AP)

    WB analysis using 12646-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12646-1-AP (PDIK1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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PDIK1L Polyclonal antibody

PDIK1L Polyclonal Antibody for WB, ELISA
Cat No. 12646-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CLIK1L, PDIK1L, RP11 96L14.4, STK35L2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat spleen tissue, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12646-1-AP targets PDIK1L in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDIK1L fusion protein Ag3356 相同性解析による交差性が予測される生物種
Full Name PDLIM1 interacting kinase 1 like
Calculated molecular weight 341 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC028713
Gene Symbol PDIK1L
Gene ID (NCBI) 149420
RRIDAB_3669146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N165
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIK1L, also known as CLIK1L, belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PDIK1L is a protein kinase associated with CLP-36-interacting kinase and is widely expressed in many organs and tissues. PDIK1L also expresses in endometriotic cells abundantly and stromal fibroblast moderately, and this protein would be released into the abdominal cavity during menstruation cycles and could elicit an auto-antibody reaction (PMID: 20403595, PMID: 33722753).

Protocols

Product Specific Protocols
WB protocol for PDIK1L antibody 12646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols