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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDIA3 Recombinant antibody (85087-5-RR)

    WB analysis using 85087-5-RR (same clone as 85087-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85087-5-RR (PDIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85087-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PDIA3 Recombinant antibody (85087-5-RR)

    IHC staining of human lung cancer using 85087-5-RR (same clone as 85087-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85087-5-RR (PDIA3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85087-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using PDIA3 Recombinant antibody (85087-5-RR)

    FC experiment of HeLa using 85087-5-RR (same clone as 85087-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDIA3 Recombinant antibody (85087-5-RR, Clone:242735H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85087-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85087-5-RR

    Affinity and Kinetic Characterization of 85087-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85087-5-RR against Human PDIA3 were performed. The affinity constant is 13.0 nM.

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PDIA3 Recombinant antibody, PBS Only

PDIA3 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 85087-5-PBS
Clone No.242735H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

ERp57, 242735H7, 58 kDa glucose-regulated protein, Disulfide isomerase ER-60, EC:5.3.4.1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85087-5-PBS targets PDIA3 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDIA3 fusion protein Ag8741 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 3
Calculated molecular weight 505 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014433
Gene Symbol PDIA3
Gene ID (NCBI) 2923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30101
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.