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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDIA2 Recombinant antibody (84765-4-RR)

    WB analysis using 84765-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84765-4-RR (PDIA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84765-4-RR

    Affinity and Kinetic Characterization of 84765-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84765-4-RR against Human PDIA2 were performed. The affinity constant is 24.0 pM.

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PDIA2 Recombinant antibody

PDIA2 Recombinant Antibody for WB, ELISA
Cat No. 84765-4-RR
Clone No.242294E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Protein disulfide isomerase A2, PDIR, PDIP, PDI, PDA2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue, mouse stomach tissue, rat stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84765-4-RR targets PDIA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDIA2 fusion protein Ag35301 相同性解析による交差性が予測される生物種
Full Name protein disulfide isomerase family A, member 2
Calculated molecular weight58 kDa
Observed molecular weight60-65 kDa
GenBank accession numberNM_006849.2
Gene Symbol PDIA2
Gene ID (NCBI) 64714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13087
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA2 (Protein disulfide-isomerase A2), a member of PDI family, is an ER protein chaperone that catalyzes the formation and breakage of disulfide bonds between cysteine residues and can inhibit aggregation of misfolded proteins by catalyzing rearrangement of -S-S- bonds in proteins. (PMID: 23486466)

Protocols

Product Specific Protocols
WB protocol for PDIA2 antibody 84765-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols