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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDHX Polyclonal antibody (10951-1-AP)

    WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDHX Polyclonal antibody (10951-1-AP)

    WB analysis using 10951-1-AP (same clone as 10951-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using PDHX Polyclonal antibody (10951-1-AP)

    WB analysis of SKOV-3 using 10951-1-AP (same clone as 10951-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PDHX Polyclonal antibody (10951-1-AP)

    WB analysis of HEK-293 using 10951-1-AP (same clone as 10951-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10951-1-AP (PDHX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PDHX Polyclonal antibody (10951-1-AP)

    IP experiment of HEK-293 using 10951-1-AP (same clone as 10951-1-PBS)

    IP result of anti-PDHX (IP:10951-1-AP, 4ug; Detection:10951-1-AP 1:5000) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PDHX Polyclonal antibody (10951-1-AP)

    IHC staining of human colon cancer using 10951-1-AP (same clone as 10951-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10951-1-AP (PDHX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10951-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PDHX Polyclonal antibody, PBS Only

PDHX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10951-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

PDX1, pyruvate dehydrogenase complex, component X, Lipoyl-containing pyruvate dehydrogenase complex component X, E3-binding protein, E3 binding protein

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10951-1-PBS targets PDHX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1389

Product name: Recombinant human PDHX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-501 aa of BC010389

Sequence: MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATVKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase complex, component X
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC010389
Gene Symbol PDHX
Gene ID (NCBI) 8050
RRIDAB_2163102
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO00330
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDHX (Pyruvate dehydrogenase protein X component), an E3-binding protein in the PDC, is acetylated by the p300 at Lys 488, impeding the interaction between PDHX and dihydrolipoyl transacetylase (E2), thereby disrupting PDC assembly to inhibit its activation (PMID: 30012170). PDHX was characterized as a metabolically essential gene for esophageal squamous cell carcinoma (ESCC), and knockdown of PDHX inhibited the proliferation of cancer stem cells (CSCs) and in vivo tumor growth (PMID: 33964039). Western blot analysis detected PDHX at an apparent molecular mass of 54 kDa.

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