Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, U2OS cells, SK-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60535-13-Ig targets PDGFR beta/CD140b in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Eg0147 Product name: Recombinant Human PDGFR beta/CD140b protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 33-531 aa of BC032224 Sequence: LVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFFGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFK 相同性解析による交差性が予測される生物種 |
| Full Name | platelet-derived growth factor receptor, beta polypeptide |
| Calculated molecular weight | 1106 aa, 124 kDa |
| Observed molecular weight | 160-180 kDa |
| GenBank accession number | BC032224 |
| Gene Symbol | PDGFR beta |
| Gene ID (NCBI) | 5159 |
| RRID | AB_3743219 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09619 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDGFR beta/CD140b antibody 60535-13-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |