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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDGFA Polyclonal antibody (26940-1-AP)

    WB analysis using 26940-1-AP (same clone as 26940-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26940-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDGFA Polyclonal antibody (26940-1-AP)

    WB analysis using 26940-1-AP (same clone as 26940-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26940-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDGFA Polyclonal antibody (26940-1-AP)

    WB analysis using 26940-1-AP (same clone as 26940-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26940-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDGFA Polyclonal antibody (26940-1-AP)

    WB analysis using 26940-1-AP (same clone as 26940-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26940-1-PBS in a different storage buffer formulation.

PDGFA Polyclonal antibody, PBS Only

PDGFA Polyclonal Antibody for WB, Indirect ELISA
Cat No. 26940-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

PDGF 1, PDGF A, PDGF AA, PDGF subunit A, PDGF1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26940-1-PBS targets PDGFA in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25643

Product name: Recombinant human PDGFA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 135-196 aa of BC146594

Sequence: TSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTDVR

相同性解析による交差性が予測される生物種
Full Name platelet-derived growth factor alpha polypeptide
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight13-16 kDa
GenBank accession numberBC146594
Gene Symbol PDGFA
Gene ID (NCBI) 5154
RRIDAB_3669576
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04085
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDGFA is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFA plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFA is also involved in oligodendrocyte development and the myelination process in the spinal cord and cerebellum. PDGF-A protein monomer is about 13-16 kDa, and PDGF-AA is a 28.5 kDa A chain homodimer.

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