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Validation Data Gallery

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  • Western Blot (WB) analysis of rat lung tissue using PDGF-BB Recombinant monoclonal antibody (87506-1-RR)

    WB analysis of rat lung using 87506-1-RR (same clone as 87506-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 87506-1-RR (PDGF-BB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PDGF-BB Recombinant monoclonal antibody (87506-1-RR)

    WB analysis of SH-SY5Y using 87506-1-RR (same clone as 87506-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 87506-1-RR (PDGF-BB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PDGF-BB Recombinant monoclonal antibody (87506-1-RR)

    WB analysis using 87506-1-RR (same clone as 87506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87506-1-RR (PDGF-BB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87506-1-PBS in a different storage buffer formulation.

  • Affinity of 87506-1-RR

    Affinity of 87506-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87506-1-RR against Mouse PDGF-BB were performed. The affinity constant is 2.21 nM.

Mouse PDGF-BB Recombinant monoclonal antibody, PBS Only

PDGF-BB Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 87506-1-PBS
Clone No.252712C5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

PDGF subunit B, PDGF-2, Pdgfb, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87506-1-PBS targets PDGF-BB in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4856

Product name: recombinant mouse PDGF-BB protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 82-190 aa of AAH23427.1

Sequence: SLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETIVTPRPVT

相同性解析による交差性が予測される生物種
Full Name platelet derived growth factor, B polypeptide
Calculated molecular weight25kd
Observed molecular weight30 kDa
GenBank accession numberAAH23427.1
Gene Symbol Pdgfb
Gene ID (NCBI) 18591
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP31240
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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