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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PDF Polyclonal antibody (24842-1-AP)

    WB analysis of MCF-7 using 24842-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PDF Polyclonal antibody (24842-1-AP)

    WB analysis of MDA-MB-453s using 24842-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MDA-MB-453s cells using PDF Polyclonal antibody (24842-1-AP)

    WB analysis of MDA-MB-453s using 24842-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDF Polyclonal antibody (24842-1-AP)

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PDF Polyclonal antibody (24842-1-AP)

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 40x lens).

PDF Polyclonal antibody

PDF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24842-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PDF, PDF1A, Polypeptide deformylase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24842-1-AP targets PDF in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18233

Product name: Recombinant human PDF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-243 aa of bc019912

Sequence: ALSLRPLWAAVPWGGAAAVGVRACSSTAAPDGVEGPALRRSYWRHLRRLVLGPPEPPFSHVCQVGDPVLRGVAAPVERAQLGGPELQRLTQRLVQVMRRRRCVGLSAPQLGVPRQVLALELPEALCRECPPRQRALRQMEPFPLRVFVNPSLRVLDSRLVTFPEGCESVAGFLACVPRFQAVQISGLDPNGEQVVWQASGWAARIIQHEMDHLQGCLFIDKMDSRTFTNVYWMKVND

相同性解析による交差性が予測される生物種
Full Name peptide deformylase (mitochondrial)
Calculated molecular weight 243 aa, 27 kDa
Observed molecular weight 60 kDa
GenBank accession numberbc019912
Gene Symbol PDF
Gene ID (NCBI) 64146
RRIDAB_2879753
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBH1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PDF antibody 24842-1-APDownload protocol
WB protocol for PDF antibody 24842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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