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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDF Polyclonal antibody

PDF Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24842-1-AP

Synonyms

PDF, PDF1A, Polypeptide deformylase

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 24842-1-AP

    WB analysis of MCF-7 using 24842-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-453s using 24842-1-AP

    WB analysis of MDA-MB-453s using 24842-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MDA-MB-453s using 24842-1-AP

    WB analysis of MDA-MB-453s using 24842-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24842-1-AP (PDF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24842-1-AP

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 24842-1-AP

    IHC staining of human breast cancer using 24842-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24842-1-AP (PDF Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inMCF-7 cells, MDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24842-1-AP is PDF Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDF fusion protein Ag18233 相同性解析による交差性が予測される生物種
Full Name peptide deformylase (mitochondrial)
Calculated molecular weight 243 aa, 27 kDa
Observed molecular weight 60 kDa
GenBank accession numberbc019912
Gene symbol PDF
Gene ID (NCBI) 64146
RRIDAB_2879753
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDF antibody 24842-1-APDownload protocol
IHC protocol for PDF antibody 24842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols