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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using PDE8A Polyclonal antibody (13956-1-AP)

    WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse spleen tissue using PDE8A Polyclonal antibody (13956-1-AP)

    WB analysis of mouse spleen using 13956-1-AP (same clone as 13956-1-PBS)

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using PDE8A Polyclonal antibody (13956-1-AP)

    WB analysis of SH-SY5Y using 13956-1-AP (same clone as 13956-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PDE8A Polyclonal antibody (13956-1-AP)

    WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PDE8A Polyclonal antibody (13956-1-AP)

    IP experiment of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

    IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PDE8A Polyclonal antibody (13956-1-AP)

    IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PDE8A Polyclonal antibody (13956-1-AP)

    IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

PDE8A Polyclonal antibody, PBS Only

PDE8A Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13956-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13956-1-PBS targets PDE8A in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5069

Product name: Recombinant human PDE8A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 530-829 aa of BC060762

Sequence: FLHCSESTLRSWLQIIEANYHSSNPYHNSTHSADVLHATAYFLSKERIKETLDPIDEVAALIAATIHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTTGDDKCNIFKNMERNDYRTLRQGIIDMVLATEMTKHFEHVNKFVNSINKPLATLEENGETDKNQEVINTMLRTPENRTLIKRMLIKCADVSNPCRPLQYCIEWAARISEEYFSQTDEEKQQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFVDLPDLMQHLDNNFKYWKGLDEMKLRNLRPPPE

相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 8A
Calculated molecular weight 93 kDa
Observed molecular weight 93 kDa, 88 kDa
GenBank accession numberBC060762
Gene Symbol PDE8A
Gene ID (NCBI) 5151
RRIDAB_2161728
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60658
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).

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