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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of Jurkat using 67565-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis using 67565-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of human placenta using 67565-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat retina tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of rat retina using 67565-1-Ig

    rat retina tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of mouse brain using 67565-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of HeLa using 67565-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PDE6D Monoclonal antibody (67565-1-Ig)

    WB analysis of HepG2 using 67565-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67565-1-Ig (PDE6D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    IHC staining of mouse brain using 67565-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat stomach tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    IHC staining of rat stomach using 67565-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using PDE6D Monoclonal antibody (67565-1-Ig)

    IHC staining of rat brain using 67565-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67565-1-Ig (PDE6D antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDE6D Monoclonal antibody

PDE6D Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67565-1-Ig
Clone No.1H4B6
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

1H4B6, PDED

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells, human placenta tissue, rat retina tissue, mouse brain tissue, JAR cells, Pig brain tissue, Rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67565-1-Ig targets PDE6D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29563

Product name: Recombinant human PDE6D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of NM_002601

Sequence: MSAKDERAREILRGFKLNWMNLRDAETGKILWQGTEDLSVPGVEHEARVPKKILKCKAVSRELNFSSTEQMEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPASVLTGNVIIETKFFDDDLLVSTSRVRLFYV

相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 6D, cGMP-specific, rod, delta
Calculated molecular weight 17 KDa
Observed molecular weight 15-17 kDa
GenBank accession numberNM_002601
Gene Symbol PDE6D
Gene ID (NCBI) 5147
RRIDAB_2882779
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDO43924
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.

Protocols

Product Specific Protocols
IHC protocol for PDE6D antibody 67565-1-IgDownload protocol
WB protocol for PDE6D antibody 67565-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Cancer Lett

Metformin inhibits the progression of castration resistant prostate cancer by regulating PDE6D induced purine metabolic alternation and cGMP / PKG pathway activation

Authors - Shanghua Cai
  • KD Validated
View Article
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