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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDE3B Polyclonal antibody

PDE3B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25290-1-AP

Synonyms

CGI PDE B, CGIP1, cGIPDE1, HcGIP1, PDE3B

Validation Data Gallery

Applications:
  • WB analysis of HL-60 using 25290-1-AP

    WB analysis of HL-60 using 25290-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25290-1-AP

    WB analysis of HeLa using 25290-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25290-1-AP

    IP experiment of HeLa using 25290-1-AP

    IP result of anti-PDE3B (IP:25290-1-AP, 5ug; Detection:25290-1-AP 1:300) with HeLa cells lysate 800ug.

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

25290-1-AP targets PDE3B in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE3B fusion protein Ag18064 相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 3B, cGMP-inhibited
Calculated molecular weight 1112 aa, 124 kDa
Observed molecular weight 124 kDa
GenBank accession numberBC136565
Gene symbol PDE3B
Gene ID (NCBI) 5140
RRIDAB_2880010
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDE3B antibody 25290-1-APDownload protocol
IP protocol for PDE3B antibody 25290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int J Mol Sci

Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions

Authors - Ning Liu
View Article