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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using PDE1C Polyclonal antibody (13785-1-AP)

    WB analysis of human heart using 13785-1-AP (same clone as 13785-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13785-1-AP (PDE1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using PDE1C Polyclonal antibody (13785-1-AP)

    IP experiment of mouse heart using 13785-1-AP (same clone as 13785-1-PBS)

    IP result of anti-PDE1C (IP:13785-1-AP, 4ug; Detection:13785-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDE1C Polyclonal antibody (13785-1-AP)

    IHC staining of human liver using 13785-1-AP (same clone as 13785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13785-1-AP (PDE1C antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using PDE1C Polyclonal antibody (13785-1-AP)

    IHC staining of human liver using 13785-1-AP (same clone as 13785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13785-1-AP (PDE1C antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13785-1-PBS in a different storage buffer formulation.

PDE1C Polyclonal antibody, PBS Only

PDE1C Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13785-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Cam PDE 1C, Cam-PDE 1C, Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C, EC:3.1.4.17, hCam 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13785-1-PBS targets PDE1C in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4798

Product name: Recombinant human PDE1C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 284-634 aa of BC022525

Sequence: RSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARMAAEEQQKEMEAKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE

相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 1C, calmodulin-dependent 70kDa
Calculated molecular weight 634 aa, 72 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC022525
Gene Symbol PDE1C
Gene ID (NCBI) 5137
RRIDAB_2268145
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14123
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. PDE1C, is calmodulin-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex. Expression of PDE1C may be an important component in signal transduction pathways mediated by calcium that permit cell cycle traverse and proliferation(PMID:9366577). It has 2 isoforms produced by alternative splicing.

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