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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using PDE12 Polyclonal antibody (12753-1-AP)

    WB analysis of human brain using 12753-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PDE12 Polyclonal antibody (12753-1-AP)

    WB analysis using 12753-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PDE12 Polyclonal antibody (12753-1-AP)

    IP experiment of mouse brain using 12753-1-AP

    IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PDE12 Polyclonal antibody (12753-1-AP)

    IF Staining of HepG2 using 12753-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using PDE12 Polyclonal antibody (12753-1-AP)

    FC experiment of HepG2 using 12753-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PDE12 Polyclonal antibody

PDE12 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12753-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

2 PDE, 2' PDE, 2',5'-phosphodiesterase 12, 2PDE, 2'PDE

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, THP-1 cells
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12753-1-AP targets PDE12 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3217

Product name: Recombinant human PDE12 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 124-472 aa of BC034978

Sequence: KLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEVGVPSSLSPSSPSSSWTETDVEERVYTPSNADIGLRLKLHCTPGDGQRFGHSRELESVCVVEAGPGTCTFDHRHLYTKKVTEDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADVICLQEVDRAVFSDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFYEALESDPLHKELLEKLVLYPSAQEKVLQRSSVLQVSVLQSTKDSSKRICVANTHLYWHPKGRFYLVSQVT

相同性解析による交差性が予測される生物種
Full Name phosphodiesterase 12
Calculated molecular weight 472 aa, 52 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC034978
Gene Symbol PDE12
Gene ID (NCBI) 201626
RRIDAB_2162883
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6L8Q7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDE12 antibody 12753-1-APDownload protocol
IF protocol for PDE12 antibody 12753-1-APDownload protocol
IP protocol for PDE12 antibody 12753-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Eur J Pharmacol

PDE12 disrupts mitochondrial oxidative phosphorylation and mediates mitochondrial dysfunction to induce oral mucosal epithelial barrier damage in oral submucous fibrosis

Authors - Qihui Wei
  • KD Validated
View Article