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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PDCL3 Polyclonal antibody

PDCL3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14997-1-AP

Synonyms

HTPHLP, PDCL3, PHLP2A, PHLP3, phosducin like 3, Phosducin like protein 3, PhPL3, VIAF, VIAF 1, VIAF1, Viral IAP associated factor 1

Validation Data Gallery

Applications:
  • WB analysis using 14997-1-AP

    WB analysis using 14997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14997-1-AP (PDCL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14997-1-AP

    WB analysis using 14997-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14997-1-AP (PDCL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 14997-1-AP

    IF Staining of HepG2 using 14997-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDCL3 antibody (14997-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 14997-1-AP

    IF Staining of HepG2 using 14997-1-AP

    Immunofluorescent analysis of HepG2 cells, using PDCL3 antibody 14997-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IF Staining of MCF-7 using 14997-1-AP

    IF Staining of MCF-7 using 14997-1-AP

    Immunofluorescent analysis of MCF-7 cells, using PDCL3 antibody 14997-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of HeLa using 14997-1-AP

    IF Staining of HeLa using 14997-1-AP

    Immunofluorescent analysis of Hela cells, using PDCL3 antibody 14997-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA2780 cells, COLO 320 cells, MCF-7 cells
Positive IF detected inHepG2 cells, MCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14997-1-AP targets PDCL3 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCL3 fusion protein Ag6983 相同性解析による交差性が予測される生物種
Full Name phosducin-like 3
Calculated molecular weight 28 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC001021
Gene symbol PDCL3
Gene ID (NCBI) 79031
RRIDAB_2878098
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PDCL3 antibody 14997-1-APDownload protocol
IF protocol for PDCL3 antibody 14997-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols