Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

human urine exosome samples were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

WB result of Alix antibody (12422-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IP result of anti-Alix (IP:12422-1-AP, 3ug; Detection:12422-1-AP 1:500) with Jurkat cells lysate 4000ug.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12422-1-AP (Alix antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12422-1-AP (Alix antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Alix antibody (12422-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1x10^6 HeLa cells were intracellularly stained with 0.25 ug Alix Polyclonal antibody (12422-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).