Validation Data Gallery

Filter: AllWBIHC

WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)
WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 66100-1-Ig (same clone as 66100-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PDCD4 Monoclonal antibody, PBS Only

PDCD4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66100-1-PBS

Clone No.1F1A11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

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MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66100-1-PBS targets PDCD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag19239 Product name: Recombinant human PDCD4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 169-469 aa of BC026104 Sequence: TPIIQEYFEHGDTNEVAEMLRDLNLGEMKSGVPVLAVSLALEGKASHREMTSKLLSDLCGTVMSTTDVEKSFDKLLKDLPELALDTPRAPQLVGQFIARAVGDGILCNTYIDSYKGTVDCVQARAALDKATVLLSMSKGGKRKDSVWGSGGGQQSVNHLVKEIDMLLKEYLLSGDISEAEHCLKELEVPHFHHELVYEAIIMVLESTGESTFKMILDLLKSLWKSSTITVDQMKRGYERIYNEIPDINLDVPHSYSVLERFVEECFQAGIISKQLRDLCPSRGRKRFVSEGDGGRLKPESY 相同性解析による交差性が予測される生物種
Full Name	programmed cell death 4 (neoplastic transformation inhibitor)
Calculated molecular weight	469 aa, 52 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC026104
Gene Symbol	PDCD4
Gene ID (NCBI)	27250
RRID	AB_2881499
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q53EL6
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.

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