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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PDCD2 Polyclonal antibody (10725-1-AP)

    WB analysis of HeLa using 10725-1-AP (same clone as 10725-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using PDCD2 Polyclonal antibody (10725-1-AP)

    WB analysis of mouse liver using 10725-1-AP (same clone as 10725-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10725-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of H7ES cells using PDCD2 Polyclonal antibody (10725-1-AP)

    WB analysis of H7ES using 10725-1-AP (same clone as 10725-1-PBS)

    H7ES cells were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10725-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PDCD2 Polyclonal antibody (10725-1-AP)

    IP experiment of HeLa using 10725-1-AP (same clone as 10725-1-PBS)

    IP result of anti-PDCD2 (IP:10725-1-AP, 4ug; Detection:10725-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10725-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PDCD2 Polyclonal antibody (10725-1-AP)

    IHC staining of human ovary tumor using 10725-1-AP (same clone as 10725-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10725-1-AP (PDCD2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10725-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10725-1-PBS targets PDCD2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, zebrafish samples.

Tested Reactivity human, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCD2 fusion protein Ag1139 相同性解析による交差性が予測される生物種
Full Name programmed cell death 2
Calculated molecular weight 39 kDa
Observed molecular weight 37-39 kDa, 45 kDa
GenBank accession numberBC008378
Gene Symbol PDCD2
Gene ID (NCBI) 5134
RRIDAB_2236895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16342
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin.