Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
15081-1-AP targets PDAP1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7155 Product name: Recombinant human PDAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC000684 Sequence: MPKGGRKGGHKGRARQYTSPEEIDAQLQAEKQKAREEEEQKEGGDGAAGDPKKEKKSLDSDESEDEEDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLDGPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERKAKDDATLSGKRMQSLSLNK 相同性解析による交差性が予測される生物種 |
| Full Name | PDGFA associated protein 1 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC000684 |
| Gene Symbol | PDAP1 |
| Gene ID (NCBI) | 11333 |
| RRID | AB_2159306 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13442 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PDAP1 (PDGFA-associated protein 1), also known as PAP, PAP1, or HASPP28 (28 kDa heat- and acid-stable phosphoprotein), is a protein-coding gene located on human chromosome 7q22.1 . The gene consists of 6 exons and encodes a 28 kDa phosphoprotein that is highly conserved across vertebrate species.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PDAP1 antibody 15081-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses | ||
Cancer Sci Pancreatic ductal adenocarcinoma cells regulated the gemcitabine-resistance function of CAFs by LINC00460 | ||
Proteomics Clin Appl SILAC-based quantitative proteomic analysis of gastric cancer secretome. | ||
Heliyon Single-cell RNA-Seq reveals the heterogeneity of fibroblasts within the tympanojugular paraganglioma microenvironment | ||
Stem Cell Res Ther Exosomal miR-181d-5p derived from distal airway stem cells inhibit apoptosis of pulmonary vascular endothelial cells in COPD mice by targeting PDAP1. |