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Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a Recom (65685-1-MR)

    FC experiment of mouse splenocytes using 65685-1-MR (same clone as 65685-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone:10F.9G2) and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 65685-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a Recom (65685-1-MR)

    FC experiment of mouse splenocytes using 65685-1-MR (same clone as 65685-1-PBS)

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone: 10F.9G2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone:10F.9G2) (black, dashed) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65685-1-PBS in a different storage buffer formulation.

Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a Recombinant Antibody, PBS Only

PD-L1/CD274 Uni-rAb® Recombinant Antibody for FC
Cat No. 65685-1-PBS
Clone No.10F.9G2

Host / Isotype

Mouse / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Cd274, PD-L1, B7 H1, PD L1, Pdcd1l1

Formulation:  PBS Only
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65685-1-PBS targets PD-L1/CD274 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen

N/A

相同性解析による交差性が予測される生物種
Full Name CD274 antigen
GenBank accession numberBC066841
Gene Symbol PD-L1
Gene ID (NCBI) 60533
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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