Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, THP-1 cells |
| Positive IHC detected in | human breast cancer tissue, human stomach cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 8 publications below |
| IF | See 3 publications below |
| FC | See 1 publications below |
Product Information
12383-1-AP targets PD-ECGF in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3052 Product name: Recombinant human PD-ECGF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC018160 Sequence: MAALMTPGTGAPPAPGDFSGEGSQGLPDPSPEPKQLPELIRMKRDGGRLSEADIRGFVAAVVNGSAQGAQIGAMLMAIRLRGMDLEETSVLTQALAQSGQQLEWPEAWRQQLVDKHSTGGVGDKVSLVLAPALAACGCKVPMISGRGLGHTGGTLDKLESIPGFNVIQSPEQMQVLLDQAGCCIVGQSEQLVPAEGILYAARDVTATVDSLPLITASILSKKLVEGLSALVVDVKFGGAAVFPNQEQARELAKTLVGVGASLGLRVAAALTAMDKPLGRCVGHALEVEEALLCMDGA 相同性解析による交差性が予測される生物種 |
| Full Name | thymidine phosphorylase |
| Calculated molecular weight | 482 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC018160 |
| Gene Symbol | Thymidine phosphorylase |
| Gene ID (NCBI) | 1890 |
| RRID | AB_2288047 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19971 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PD-ECGF antibody 12383-1-AP | Download protocol |
| WB protocol for PD-ECGF antibody 12383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Proteomic profiling of gliomas unveils immune and metabolism-driven subtypes with implications for anti-nucleotide metabolism therapy | ||
J Exp Clin Cancer Res Therapeutic synergies that overcome carboplatin resistance in triple-negative breast cancer. | ||
Adv Sci (Weinh) Spatial Transcriptomics Reveals Transcriptomic and Immune Microenvironment Reprogramming during Thyroid Carcinoma Dedifferentiation. | ||
Int J Biol Sci Comprehensive Metabolomic Profiling of Skin Lesions from Psoriasis Patients Reveals Disease Signatures. | ||
Acta Pharmacol Sin Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies. | ||
Oncogene The proteomic characterization of the peritumor microenvironment in human hepatocellular carcinoma. |