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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCYT1A Polyclonal antibody (30991-1-AP)

    WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PCYT1A Polyclonal antibody (30991-1-AP)

    WB analysis using 30991-1-AP (same clone as 30991-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30991-1-AP (PCYT1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PCYT1A Polyclonal antibody (30991-1-AP)

    IF Staining of A431 using 30991-1-AP (same clone as 30991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PCYT1A Polyclonal antibody (30991-1-AP)

    IF Staining of U-251 using 30991-1-AP (same clone as 30991-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PCYT1A antibody (30991-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30991-1-PBS in a different storage buffer formulation.

PCYT1A Polyclonal antibody, PBS Only

PCYT1A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30991-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CCT A, CCT alpha, CT A, CTPCT, PCYT1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30991-1-PBS targets PCYT1A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34268

Product name: Recombinant human PCYT1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-76 aa of NM_005017

Sequence: MDAQCSAKVNARKRRKEAPGPNGATEEDGVPSKVQRCAVGLRQPAPFSDEIEVDFSKPYVRVTMEEASRGTPCERP

相同性解析による交差性が予測される生物種
Full Name phosphate cytidylyltransferase 1, choline, alpha
Calculated molecular weight42 kDa
Observed molecular weight40-41 kDa
GenBank accession numberNM_005017
Gene Symbol PCYT1A
Gene ID (NCBI) 5130
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP49585
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCYT1A (phosphate cytidylyltransferase 1A, choline), also known as CTPCT. It is expected to be located in the nucleus and cytoplasm, which is enriched in brain, placenta, liver, fetal and adult lung. This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. The molecular weight of PCYT1A is 41 kDa.

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