Validation Data Gallery
Tested Applications
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18868-1-AP targets PCYOX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4316 Product name: Recombinant human PCYOX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 155-505 aa of BC033815 Sequence: WVEDVLDKFMRIYRYQSHDYAFSSVEKLLHALGGDDFLGMLNRTLLETLQKAGFSEKFLNEMIAPVMRVNYGQSTDINAFVGAVSLSCSDSGLWAVEGGNKLVCSGLLQASKSNLISGSVMYIEEKTKTKYTGNPTKMYEVVYQIGTETRSDFYDIVLVATPLNRKMSNITFLNFDPPIEEFHQYYQHIVTTLVKGELNTSIFSSRPIDKFGLNTVLTTDNSDLFINSIGIVPSVREKEDPEPSTDGTYVWKIFSQETLTKAQILKLFLSYDYAVKKPWLAYPHYKPPEKCPSIILHDRLYYLNGIECAASAMEMSAIAAHNAALLAYHRWNGHTDMIDQDGLYEKLKTEL 相同性解析による交差性が予測される生物種 |
| Full Name | prenylcysteine oxidase 1 |
| Calculated molecular weight | 505 aa, 57 kDa |
| GenBank accession number | BC033815 |
| Gene Symbol | PCYOX1 |
| Gene ID (NCBI) | 51449 |
| RRID | AB_10598471 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHG3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PCYOX1(Prenylcysteine oxidase 1) is also named as KIAA0908, PCL1, prenylcysteine lyase and belongs to the prenylcysteine oxidase family. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and four potential N-glycosylation sites. It is a glycosylated enzyme involved in prenylated protein catabolism, releasing a free cysteine and a hydrophobic isoprenoid product. Immunofluorescence studies of PCYOX1 showed a nonnuclear punctate pattern, and PCYOX1 colocalized with lysosomal marker LAMP1 in HEK293 cells(PMID:10585463). The enzyme is isolated from bovine brain membranes and exhibits an apparent molecular mass of 63 kDa(PMID:9287348).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PCYOX1 antibody 18868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
