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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using PCSK9 Recombinant antibody (83004-4-RR)

    IF Staining of mouse liver using 83004-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using PCSK9 Recombinant antibody (83004-4-RR)

    IF Staining of mouse liver using 83004-4-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PCSK9 Recombinant antibody (83004-4-RR)

    IF Staining of HepG2 using 83004-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HeLa cells using PCSK9 Recombinant antibody (83004-4-RR)

    FC experiment of HeLa using 83004-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83004-4-RR

    Affinity and Kinetic Characterization of 83004-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

PCSK9 Recombinant monoclonal antibody

PCSK9 Uni-rAb® Recombinant Antibody for IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 83004-4-RR
Clone No.230536E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

230536E4, AI415265, AI747682, FH3, HCHOLA3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83004-4-RR targets PCSK9 in IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0640

Product name: Recombinant Mouse PCSK9 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-10*His

Domain: 35-694 aa of NM_153565.2

Sequence: QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ

相同性解析による交差性が予測される生物種
Full Name proprotein convertase subtilisin/kexin type 9
Calculated molecular weight75KD
GenBank accession numberNM_153565.2
Gene Symbol Pcsk9
Gene ID (NCBI) 100102
RRIDAB_3670749
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ80W65
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).

Protocols

Product Specific Protocols
FC protocol for PCSK9 antibody 83004-4-RRDownload protocol
IF protocol for PCSK9 antibody 83004-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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