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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PCP4L1 Polyclonal antibody (25933-1-AP)

    WB analysis of mouse brain using 25933-1-AP (same clone as 25933-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PCP4L1 Polyclonal antibody (25933-1-AP)

    WB analysis of mouse brain using 25933-1-AP (same clone as 25933-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25933-1-AP (PCP4L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCP4L1 Polyclonal antibody (25933-1-AP)

    IHC staining of mouse brain using 25933-1-AP (same clone as 25933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25933-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PCP4L1 Polyclonal antibody (25933-1-AP)

    IHC staining of mouse brain using 25933-1-AP (same clone as 25933-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25933-1-AP (PCP4L1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25933-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PCP4L1 Polyclonal antibody, PBS Only

PCP4L1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25933-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25933-1-PBS targets PCP4L1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23061

Product name: Recombinant human PCP4L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of BC028905

Sequence: MSELNTKTSPATNQAAGQEEKGKAGNVKKAEEEEEIDIDLTAPETEKAALAIQGKFRRFQKRKKDPSS

相同性解析による交差性が予測される生物種
Full Name Purkinje cell protein 4 like 1
Calculated molecular weight 68 aa, 7 kDa
Observed molecular weight 8-15 kDa
GenBank accession numberBC028905
Gene Symbol PCP4L1
Gene ID (NCBI) 654790
RRIDAB_2880301
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDA6NKN8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCP4L1 (Purkinje cell protein 4-like 1) is a small neuronal IQ motif protein closely related to the calmodulin-binding protein Pcp4/PEP-19 (PMID: 22458599).

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