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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PCP2 Polyclonal antibody

PCP2 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13774-1-AP

Synonyms

GPSM4, L7, PCP2, Purkinje cell protein 2

Validation Data Gallery

Applications:
  • IHC staining of mouse cerebellum using 13774-1-AP

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 13774-1-AP

    IHC staining of mouse cerebellum using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 13774-1-AP

    IHC staining of mouse eye using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 13774-1-AP

    IHC staining of mouse eye using 13774-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13774-1-AP (PCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 13774-1-AP

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 13774-1-AP

    IF Staining of mouse cerebellum using 13774-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PCP2 antibody (13774-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse cerebellum tissue, mouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13774-1-AP targets PCP2 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCP2 fusion protein Ag4759 相同性解析による交差性が予測される生物種
Full Name Purkinje cell protein 2
Calculated molecular weight 136 aa, 15 kDa
GenBank accession numberBC038715
Gene symbol PCP2
Gene ID (NCBI) 126006
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pcp2 is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons, and it may function as a cell-type specific modulator for G protein-mediated cell signaling.

Protocols

Product Specific Protocols
IHC protocol for PCP2 antibody 13774-1-APDownload protocol
IF protocol for PCP2 antibody 13774-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols