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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of mouse testis using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of mouse testis using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of human prostate cancer using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PCOTH Polyclonal antibody (24564-1-AP)

    IHC staining of human prostate cancer using 24564-1-AP (same clone as 24564-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24564-1-AP (PCOTH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PCOTH Polyclonal antibody (24564-1-AP)

    IF Staining of PC-3 using 24564-1-AP (same clone as 24564-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PCOTH antibody (24564-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24564-1-PBS in a different storage buffer formulation.

PCOTH Polyclonal antibody, PBS Only

PCOTH Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 24564-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24564-1-PBS targets PCOTH in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20092

Product name: Recombinant human PCOTH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 8-107 aa of BC142999

Sequence: GTSEEGNLLSTVSPTVKALFGKTRVSPIFPFSPRSPFQPLIPRTPGSPWGPVGPASPLGPGFPIGPMGPGKPVGPKGPMLPLGPSGPVGPTSPLFPFCP

相同性解析による交差性が予測される生物種
Full Name prostate collagen triple helix
Calculated molecular weight 107 aa, 11 kDa
GenBank accession numberBC142999
Gene Symbol PCOTH
Gene ID (NCBI) 542767
RRIDAB_2918084
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ58A44
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

PCOTH may be involved in growth and survival of prostate cancer cells through the TAF-Ibeta pathway.