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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PCOLCE2 Polyclonal antibody (10607-1-AP)

    WB analysis of MCF-7 using 10607-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10607-1-AP (PCOLCE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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PCOLCE2 Polyclonal antibody

PCOLCE2 Polyclonal Antibody for WB, ELISA
Cat No. 10607-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

PCOLCE2, PCPE 2, PCPE2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

10607-1-AP targets PCOLCE2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCOLCE2 fusion protein Ag0879 相同性解析による交差性が予測される生物種
Full Name procollagen C-endopeptidase enhancer 2
Calculated molecular weight 46 kDa
GenBank accession numberBC006265
Gene Symbol PCOLCE2
Gene ID (NCBI) 26577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Procollagen C-endopeptidase enhancer 2 (PCOLCE2) is a 415-amino acid protein containing an N-terminal signal sequence, two CUB domains, and an NTR domain. PCOLCE2 binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. It has been reported that PCOLCE2 is a glycoprotein with an apparent molecular weight of 52 kDa, which is larger than its calculated molecular weight of 45.7 kDa (PMID: 10873381).

Protocols

Product Specific Protocols
WB protocol for PCOLCE2 antibody 10607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

PLoS Biol

Colon stroma mediates an inflammation-driven fibroblastic response controlling matrix remodeling and healing.

Authors - Guadalupe J Jasso
View Article