Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
10607-1-AP targets PCOLCE2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0879 Product name: Recombinant human PCOLCE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-276 aa of BC006265 Sequence: MRGANAWAPLCLLLAAATQLSRQQSPERPVFTCGGILTGESGFIGSEGFPGVYPPNSKCTWKITVPEGKVVVLNFRFIDLESDNLCRYDFVDVYNGHANGQRIGRFCGTFRPGALVSSGNKMMVQMISDANTAGNGFMAMFSAAEPNERGDQYCGGLLDRPSGSFKTPNWPDRDYPAGVTCVWHIVAPKNQLIELKFEKFDVERDNYCRYDYVAVFNGGEVNDARRIGKYCGDSPPAPIVSERNELLIQFLSDLSLTADGFIGHYIFRPKKLPTTT 相同性解析による交差性が予測される生物種 |
| Full Name | procollagen C-endopeptidase enhancer 2 |
| Calculated molecular weight | 46 kDa |
| GenBank accession number | BC006265 |
| Gene Symbol | PCOLCE2 |
| Gene ID (NCBI) | 26577 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Procollagen C-endopeptidase enhancer 2 (PCOLCE2) is a 415-amino acid protein containing an N-terminal signal sequence, two CUB domains, and an NTR domain. PCOLCE2 binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1. It has been reported that PCOLCE2 is a glycoprotein with an apparent molecular weight of 52 kDa, which is larger than its calculated molecular weight of 45.7 kDa (PMID: 10873381).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PCOLCE2 antibody 10607-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
